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. 2010 Feb 10;298(5):C1018–C1028. doi: 10.1152/ajpcell.00492.2009

Fig. 1.

Fig. 1.

Characterization of new NBCn1 antibodies. A: immunoblot of membrane preparation from rat whole brain. Immunoblot was performed with the affinity-purified rabbit NBCn1 antibody. The antibody detected a major band of 130 kDa. The detection of the band of 250 kDa varied depending on the amount of SDS in the sample buffer. B: immunoblot of membrane preparation from rat hippocampus with the affinity-purified guinea pig NBCn1 antibody. The band in preserum is nonspecific. C: immunocytochemistry of HEK-293 cells transfected with NBCn1. Experiments were done with the guinea pig antibody. Controls were cells transfected with vector only. Bar: 50 μm.