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. 2010 Feb 10;298(5):C1018–C1028. doi: 10.1152/ajpcell.00492.2009

Fig. 4.

Fig. 4.

Interaction between NBCn1 and PSD-95. A: GST pull-down assay. Brain lysates were incubated with the GST/NBCn1 fusion protein containing the COOH-terminal 26 amino acids of rat NBCn1, or GST only. Proteins bound to GST/NBCn1 or GST were dissociated and examined with the PSD-95 antibody by immunoblot. Xenopus oocyte lysates were used as a negative control. B: coimmunoprecipitation of NBCn1 with the mouse PSD-95 antibody. Brain lysates were incubated with the PSD-95 antibody and immunoprecipitates were examined with the NBCn1 antibody by immunoblot. Mouse IgG was used as a control.