NBCn1, NR2A, PSD-95, and β-actin expression levels before and after NMDA administration. A–D: immunoblot of the hippocampus prepared from controls and acidotic rats 3 days after NMDA treatment (n = 4). The blots were probed with antibodies to NBCn1 (A), NR2A (B), PSD-95 (C), and β-actin (D). E: quantitative measurements of immunoreactive bands. The bands were quantitated and normalized to β-actin.