Fig. 3.

Validation of Mustn1 silencing. The expression level of Mustn1 mRNA from day 4 differentiated C2C12 cells was detected by Q-PCR. All values were normalized to that of the parental cell line. Mustn1 is expressed at normal level in the parental and control [green fluorescent protein (GFP)-RNA interference (RNAi) treated] cell lines, but in lower amounts in the Mustn1 (Mustn1-RNAi treated) cells. Error bars indicate SD (n = 3). *P < 0.05, **P < 0.01, determined by one-way ANOVA with Tukey's post hoc test.