Mustn1 silencing suppresses myogenic differentiation and myotube formation. A: average number of myotubes between the parental, control, and Mustn1 RNAi cultures at 4 and 6 days following stimulation of differentiation. Error bars indicate SD (n = 5). **P < 0.01, ***P < 0.001, determined by one-way ANOVA with Tukey's post hoc test. B: photomicrographs showing the presence of myotubes in the three cell lines evaluated at day 6. Scale bar, 100 μm.