Fig. 6.

Mustn1 silencing leads to downregulation of myogenic related gene expression. RNA from control (GFP) and Mustn1 RNAi-treated cells was isolated at 48 and 96 h after plating and subjected to Q-PCR. Genes tested represented myogenic differentiation markers [myogenin (Myog) desmin (Des), and Myh4] and myofusion-related genes [calpain 1 (Capn1), caveolin 3 (Cav3), and cadherin 14 (Cadh15); (9)]. Error bars indicate SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, determined by one-way ANOVA with Tukey's post hoc test.