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Mustn1 silencing decreases Myog expression during myogenic differentiation. The parental (A and B), control (C and D), and Mustn1 (E and F) RNAi-treated C2C12 cells were seeded on coverglasses and stained at day 2 (A, C, and E) and day 4 (B, D, and F) with an anti-myogenin antibody. White arrows indicate Myog-positive nuclei within individual myotubes. Black arrows denote the absence of Myog expression. Scale bar, 10 μm.
