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Mustn1 silencing leads to downregulation of Myod gene expression. RNA from control (GFP) and Mustn1 RNAi-treated cells was isolated at 48 and 96 h after plating and subjected to Q-PCR. Aside from Myod, Myf5 was also tested but did not reveal any differences in mRNA expression between the two different cell lines. Error bars indicate SD (n = 3). *P < 0.05, **P < 0.01, determined by one-way ANOVA with Tukey's post hoc test.
