. 2010 Feb 3;298(5):C1100–C1108. doi: 10.1152/ajpcell.00553.2009

Table 1.

Primers and conditions used for quantitative real-time PCR

Target Gene	Accession No.	Primer Sequence	Amplicon Size, bp	T_m, °C
18S	AY248756	Forward: `CGCGGTTCTATTTTGTTGGT`	219	60
		Reverse: `AGTCGGCATCGTTTATGGTC`
β-Actin	NM_007393	Forward: `AGACCTTCAACACCCCAG`	166	60
		Reverse: `AGGTCCAGACGCAGGATG`
Capn1	NM_007600	Forward: `GGTGAAGTGGAGTGGAAAGG`	226	60
		Reverse: `TGCCCTCGTAAAATGTGGTA`
Cav3	NM_007617	Forward: `ACGGTGTATGGAAGGTGAGC`	203	60
		Reverse: `TGAGTAGATGTGGCTGATGC`
Des	NM_010043	Forward: `GCGGCTAAGAACATCTCTGA`	116	60
		Reverse: `TCCATCATCTCCTGCTTGG`
Cadh15	NM_007662	Forward: `CCCAACTAAGGGGCTCTCTC`	150	60
		Reverse: `ATTCTCCCACCACTCCTGACT`
Mustn1	NM_181390	Forward: `AAGAAGAAGCGGCCCCCT`	190	60
		Reverse: `CTTTGGGCTTCTCAAAGAC`
Myh4	NM_010855	Forward: `CAAGTCATCGGTGTTTGTGG`	175	60
		Reverse: `GGCCATGTCCTCAATCTTGT`
Myod	NM_010866	Forward: `GCCTGAGCAAAGTGAATGAG`	184	60
		Reverse: `GGTCCAGGTGCGTAGAAGG`
Myog	NM_031189	Forward: `GGAAGTCTGTGTCGGTGGAC`	150	60
		Reverse: `CGCTGCGCAGGATCTCCAC`

T_m, temperature.