Fig. 4.

PKA inhibitor mPKI prevents apical V-ATPase accumulation in kidney intercalated cells. Confocal images of V-ATPase E subunit immunofluorescence labeling in kidney slices incubated either in Ringer buffer for 75 min (A) or in the presence of the specific PKA inhibitor mPKI (10 μM; B). Quantification of the mean (±SE) V-ATPase-associated MPI apical-to-cytoplasmic ratio under the different conditions demonstrates that mPKI prevented apical membrane accumulation (*P < 0.05 vs. Ringer buffer control, 75 min; C). Scale bar = 10 μm.