Fig. 3.

Tubulointerstitial fibrosis precedes glomerulosclerosis in the remnant kidney after nephrectomy and is prevented by caloric restriction. Paraffin sections of remnant kidneys of rats 4 wk after nephrectomy by the infarction method, perfusion fixed with formaldehyde, and stained with hematoxylin and eosin. Rats were fed a 21% casein-based diet ad libitum (right) or pair fed a 35% casein-based diet at 60% of total food weight consumed by the ad libitum group, so that both groups consumed equal amounts of protein. The ad libitum group, but not the calorie-restricted group will develop end-stage renal disease after 5 mo (148). Original magnification: ×200. Unpublished archival material from Ref. 85.