Fig. 4.

Increased ASIC3 and ASIC2a protein expression in urothelium with CYP-induced cystitis (4 h, 48 h, and chronic). A: Western blot of urothelium (20 μg) for ASIC3 expression in control rats and those treated with CYP for 4 h, 48 h, and 10 days (chronic). Actin expression was used as a loading control. B: relative urothelium ASIC3 band density in all groups examined normalized to actin in the same samples presented as a percentage of control ASIC3 expression. C: Western blot of urothelium (20 μg) for ASIC2a expression in control rats and those treated with CYP for 4 h, 48 h, and 10 days (chronic). Actin expression was used as a loading control. D: relative urothelium ASIC2a band density in all groups examined normalized to actin in the same samples presented as a percentage of control ASIC2a expression. *P ≤ 0.01 vs. control or as indicated between groups by horizontal lines (n = 5–6 for each group).