Table 2.
Secondary Antibody | Source | Primary Antibody Reacted With | Working Dilution | Wavelength Used |
---|---|---|---|---|
Alexa-Fluor goat anti-mouse | Molecular Probes, Eugene, OR | Anti-KIT (Ab-4; cocktail) | 1:1,000 | 594 nm |
Alexa-Fluor goat anti-rabbit | Molecular Probes | Anti-KIT (Human CD117/KIT) | 1:1,000 | 594 nm |
Alexa-Fluor goat anti-rabbit | Molecular Probes | Anti-tyrosine hydroxylase; anti-protein gene product 9.5; anti-smooth muscle myosin | 1:1,000 | 488 nm |
Alexa-Fluor donkey anti-sheep | Molecular Probes | Anti-nNOS | 1:1,000 | 488 nm |