Table 1.

Effect of chronic ethanol consumption and SAM on liver SAM, SAH, and SAM/SAH ratio

Tissue/Treatment	SAM	SAH	SAM/SAH ratio
Liver*
Control	2.09 ± 0.17	0.54 ± 0.04	3.9 ± 0.22
Ethanol	1.62 ± 0.13	0.73 ± 0.06	2.2 ± 0.05
Control + SAM	2.09 ± 0.10	0.58 ± 0.05	3.8 ± 0.42
Ethanol + SAM	1.95 ± 0.09	0.88 ± 0.08	2.3 ± 0.22
ANOVA (P values)†
Ethanol effect	0.029	0.0007	<0.0001
SAM effect	0.19	0.14	0.94
Interaction (Ethanol × SAM)	0.22	0.37	0.69
Mitochondria‡
Control	0.037 ± 0.002	0.010 ± 0.0003	3.6 ± 0.17
Ethanol	0.057 ± 0.005	0.014 ± 0.001	4.1 ± 0.21
Control + SAM	0.054 ± 0.010	0.009 ± 0.001	5.7 ± 0.67
Ethanol + SAM	0.082 ± 0.004	0.013 ± 0.001	6.5 ± 0.46
ANOVA (P values)§
Ethanol effect	0.0008	0.0027	0.138
SAM effect	0.0022	0.31	<0.0001
Interaction (Ethanol × SAM)	0.542	0.98	0.81

Data are represented as means ± SE for n = 5 animals per treatment group.

^*Liver concentrations of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are expressed as nmol·liver⁻¹·g body wt⁻¹ (8).

^†,§Results from 2-factor ANOVA with ethanol effect (control vs. ethanol), SAM effect (absent vs. present), and interaction (Ethanol × SAM). †Liver values and

^§mitochondria values.

^‡Mitochondrial concentrations of SAM and SAH are expressed as nmol/mg protein.