Table 4.
Spot No.* | Protein | Mass, kDa | Ethanol Effect† | SAM Effect† | Interaction (E×SAM)† | C vs. E‡ | C vs. SAM‡ | SAM vs. E+SAM‡ | E vs. E+SAM‡ |
---|---|---|---|---|---|---|---|---|---|
1 | NADH dehydrogenase Fe-S Protein 1 | 79.7 | P = 0.003 | P = 0.04 | P = 0.002 | ||||
2 | NADH dehydrogenase flavoprotein 1 | 50.8 | P = 0.01 | P = 0.05 | |||||
3 | NADH dehydrogenase Fe-S protein 2 | 53.3 | P = 0.04 | P = 0.05 | |||||
4 | NADH dehydrogenase 1α sub- complex 10 | 40.6 | |||||||
5 | NADH dehydrogenase flavoprotein 2 | 27.3 | |||||||
6 | NADH dehydrogeanse 1 α sub-complex 8 | 20.0 | P = 0.0002 | P = 0.05 | P = 0.01 | P = 0.005 | |||
7 | NADH dehydrogenase 1 α sub-complex subunit 12 | 17.1 | P = 0.005 | ||||||
8 | NADH dehydrogenase 1 β sub-complex 4 | 15.1 | P = 0.04 | ||||||
9 | NADH dehydrogenase Fe-S protein 6 | 13.0 | P = 0.04 | ||||||
10 | ATP synthase, H+ transporting F1 complex α subunit | 59.7 | |||||||
ATP synthase, H+ transporting F1 complex β subunit | 48.1 | ||||||||
11 | ATP synthase, γ chain | 32.9 | P = 0.05 | ||||||
12 | ATP synthase, H+ transporting F0 complex subunit b isoform 1 | 28.9 | |||||||
13 | ATP synthase, H+ transporting F0 complex subunit d | 18.7 | P = 0.03 | P = 0.04 | |||||
14 | Cytochrome c oxidase, subunit IV isoform1 | 19.5 | P = 0.02 | P = 0.05 | |||||
15 | ATP synthase, H+ transporting F0 complex subunit g | 11.4 | P = 0.04 | P = 0.009 | |||||
16 | Mitochondrial trifunctional protein α subunit | 82.6 | |||||||
17 | 60 kDa heat shock protein, mitochondrial | 61.0 | |||||||
18 | Ubiquinol-cytochrome c reductase, core protein 1 | 52.7 | |||||||
19 | Ubiquinol-cytochrome c reductase, core protein 2 | 48.2 | |||||||
20 | Cytochrome c1 | 29.5 | |||||||
21 | Ubiquinol-cytochrome c reductase, Rieske Fe-S polypeptide 1 | 29.3 | |||||||
22 | Ubiquinol-cytochrome c reductase, subunit 7 | 13.5 | P = 0.05 | ||||||
23 | Ubiquinol-cytochrome c reductase, subunit 8 | 9.8 | P = 0.02 | P = 0.05 | P = 0.05 | ||||
24 | Ubiquinol-cytochrome c reductase, subunit 9 | 7.4 | |||||||
25 | 2-hydroxyacyl-CoA lyase | 63.6 | |||||||
26 | Electron transferring flavoprotein β | 27.6 | |||||||
27 | Cytochrome c oxidase, subunit II | 25.8 | P = 0.001 | P = 0.004 | P = 0.02 | P = 0.04 | |||
28 | Unidentified§ | P = 0.008 | P = 0.002 | ||||||
29 | Cytochrome c oxidase, subunit IV isoform 1 | 19.5 | P = 0.04 | ||||||
30 | Cytochrome c oxidase, subunit Va | 16.0 | P = 0.05 | ||||||
31 | Cytochrome c oxidase, subunit Vla | 12.3 | P = 0.03 | P = 0.02 | |||||
32 | Cytochrome c oxidase, subunit VIb polypeptide 1 | 10.1 | P = 0.009 | ||||||
33 | Unidentified‡ | 6.0 | P = 0.05 | P = 0.007 |
Oxidative phosphorylation and associated proteins listed above were matched on all 2D blue native gel electrophoresis (BN-PAGE) gels from the control, ethanol, control + SAM, and ethanol + SAM groups.
Spot no. is the same as those used to identify protein spots in Fig. 6.
Two-factor ANOVA P value.
Student's t-test P value.
Unable to identify probable cytochrome c oxidase subunits. Note that not all proteins listed in this table were altered in abundance by ethanol, SAM, or in combination but are included for protein identification. Molecular weight search (MOWSE) scores and accession numbers are listed in the table provided in supplemental data online.