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. 2010 Feb 11;298(5):G732–G745. doi: 10.1152/ajpgi.00332.2009

Table 4.

Oxidative phosphorylation and associated proteins shown in Fig. 6 that are differentially altered in abundance by chronic ethanol and/or SAM treatments: results from BN-PAGE

Spot No.* Protein Mass, kDa Ethanol Effect SAM Effect Interaction (E×SAM) C vs. E C vs. SAM SAM vs. E+SAM E vs. E+SAM
1 NADH dehydrogenase Fe-S Protein 1 79.7 P = 0.003 P = 0.04 P = 0.002
2 NADH dehydrogenase flavoprotein 1 50.8 P = 0.01 P = 0.05
3 NADH dehydrogenase Fe-S protein 2 53.3 P = 0.04 P = 0.05
4 NADH dehydrogenase 1α sub- complex 10 40.6
5 NADH dehydrogenase flavoprotein 2 27.3
6 NADH dehydrogeanse 1 α sub-complex 8 20.0 P = 0.0002 P = 0.05 P = 0.01 P = 0.005
7 NADH dehydrogenase 1 α sub-complex subunit 12 17.1 P = 0.005
8 NADH dehydrogenase 1 β sub-complex 4 15.1 P = 0.04
9 NADH dehydrogenase Fe-S protein 6 13.0 P = 0.04
10 ATP synthase, H+ transporting F1 complex α subunit 59.7
ATP synthase, H+ transporting F1 complex β subunit 48.1
11 ATP synthase, γ chain 32.9 P = 0.05
12 ATP synthase, H+ transporting F0 complex subunit b isoform 1 28.9
13 ATP synthase, H+ transporting F0 complex subunit d 18.7 P = 0.03 P = 0.04
14 Cytochrome c oxidase, subunit IV isoform1 19.5 P = 0.02 P = 0.05
15 ATP synthase, H+ transporting F0 complex subunit g 11.4 P = 0.04 P = 0.009
16 Mitochondrial trifunctional protein α subunit 82.6
17 60 kDa heat shock protein, mitochondrial 61.0
18 Ubiquinol-cytochrome c reductase, core protein 1 52.7
19 Ubiquinol-cytochrome c reductase, core protein 2 48.2
20 Cytochrome c1 29.5
21 Ubiquinol-cytochrome c reductase, Rieske Fe-S polypeptide 1 29.3
22 Ubiquinol-cytochrome c reductase, subunit 7 13.5 P = 0.05
23 Ubiquinol-cytochrome c reductase, subunit 8 9.8 P = 0.02 P = 0.05 P = 0.05
24 Ubiquinol-cytochrome c reductase, subunit 9 7.4
25 2-hydroxyacyl-CoA lyase 63.6
26 Electron transferring flavoprotein β 27.6
27 Cytochrome c oxidase, subunit II 25.8 P = 0.001 P = 0.004 P = 0.02 P = 0.04
28 Unidentified§ P = 0.008 P = 0.002
29 Cytochrome c oxidase, subunit IV isoform 1 19.5 P = 0.04
30 Cytochrome c oxidase, subunit Va 16.0 P = 0.05
31 Cytochrome c oxidase, subunit Vla 12.3 P = 0.03 P = 0.02
32 Cytochrome c oxidase, subunit VIb polypeptide 1 10.1 P = 0.009
33 Unidentified 6.0 P = 0.05 P = 0.007

Oxidative phosphorylation and associated proteins listed above were matched on all 2D blue native gel electrophoresis (BN-PAGE) gels from the control, ethanol, control + SAM, and ethanol + SAM groups.

*

Spot no. is the same as those used to identify protein spots in Fig. 6.

Two-factor ANOVA P value.

Student's t-test P value.

§

Unable to identify probable cytochrome c oxidase subunits. Note that not all proteins listed in this table were altered in abundance by ethanol, SAM, or in combination but are included for protein identification. Molecular weight search (MOWSE) scores and accession numbers are listed in the table provided in supplemental data online.