Fig. 3.

Intestinal reactive oxygen species (ROS) accumulation and zinc concentrations in mice chronically fed alcohol for 4 wk. A: fluorescence microscopy of ROS. Cryostat intestinal sections were incubated with dihydroethidium (DHE) at 5 μM, and red fluorescence was formed in the nuclei upon oxidation by ROS. Scale bar = 40 μM. B: quantitative analysis of the ethidium fluorescence intensity. C: intestinal zinc concentrations. Total zinc was measured by atom absorption spectroscopy. Results are means ± SD (n = 6–8). *Significantly different (P < 0.05, t-test) from the pair-fed mice.