Fig. 3.

Effect of MP treatment on ASBT in villus cell brush-border membrane vesicle (BBMV) isolated from the normal and chronically inflamed ileum. Na-dependent taurocholate uptake (pmol/mg of protein) is defined as taurocholate uptake in the presence of extravesicular Na minus that in the absence of Na. Y-axis labeling is the same for A and B. Statistical comparisons are made of uptakes of different conditions for each time point. All uptakes were done in triplicate, and each n represents BBMV preparations from different animals. Statistical comparisons are made of uptakes of different conditions for each time point. A: Na-dependent taurocholate uptake as a function of time in villus cell BBMV from control rabbits. This uptake was significantly increased at all time points measured in villus cell BBMV from MP-treated normal rabbits. B: in villus cell BBMV from the chronically inflamed ileum, Na-dependent taurocholate uptake was significantly reduced. Treatment of rabbits with chronic ileitis with MP almost completely reversed the inhibition of Na-dependent taurocholate uptake.