Fig. 2.

Wortmannin does not inhibit cAMP-induced p38 MAPK activation in rat hepatocytes. Hepatocytes were treated with or without 50 nm wortmannin (Wort) for 15 min before addition of 100 μM CPT-cAMP or buffer. Cell lysates prepared 15 min after the addition of buffer (control) or CPT-cAMP (cAMP) were analyzed for phosphorylated (Phospho) and total p38 MAPK and PKB. Top: representative immunoblots. Bottom: results of densitometric analysis (means ± SE; n = 3). *Significantly (P < 0.05) different from respective control values.