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p38α MAPK shRNA decreases expression of p38α but not p38β MAPK. HuH-7 cells transfected with scrambled (Scr) shRNA or p38α MAPK shRNA were treated with or without 100 μM CPT-cAMP for 15 min followed by immunoblotting of p38α or p38β MAPK with actin as loading control. Top: representative immunoblots. Bottom: results of densitometric analysis (means ± SE; n = 3). *Significantly (P < 0.05) different from respective scrambled shRNA controls in the absence of cAMP.
