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. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: J Environ Prot Sci. 2007 Jan 1;1:23–28.

Figure 1.

Figure 1

Measurement of fluorescent signal for Hoechst 33342 and dBBr in HEK293T cells as a function of increased starting cell density. (A) Hoechst 33342 signal at [λex340 nm, λem450 nm; open circles O], and at [λex 393 nm, λem450 nm; closed circles ●] in HEK293T cells (B) Dibromobimane signal at [λex 393 nm, λem 477 nm; open circles O], and at [λex340 nm, λem 477 nm; closed circles ●] in HEK293T cells. (C) Changes in dBBr fluorescence normalized to the Hoechst 33342 as a function of increasing cell density in HEK293T cells; dBBr fluorescent arbitrary units (F.A.U.) were normalized by the Hoechst 33342 F.A.U. of the same well. Values were calculated by the following formula; [dBBr F.A.U./Hoechst F.A.U.] and reported as mean ± SEM.