Table 2.

Characteristics of primers

Gene Namea (abb.)	Gene Symbol	Primer Sequenceb	Accession Numberc	Amplicon Sizesd, bp	Annealing Tme, °C
Potassium large-conductance calcium-activated channel, subfamily M, α member 1 (BK-α)	Kcnma1	F:AGGCTCTGTTCAAACGGCAT	NM_031828	250	58
		R:TTGAGCAGATGGGCCTTGTT
Potassium large-conductance calcium-activated channel, subfamily M, β member 1 (BK-β1)	Kcnmb1	F:ACCCATGCCTTTGGGTCAAT	NM_019273	236	58
		R:ATAGAGGCGCTGGTACACAA
Potassium large-conductance calcium-activated channel, subfamily M, β member 2 (BK-β2)	Kcnmb2	F:TGCCCTGCTGAATGTGTCAA	NM_176861	231	58
		R:ACACTCACAAGGGACATGGA
Potassium large-conductance calcium-activated channel, subfamily M, β member 4 (BK-β4)	Kcnmb4	F:TCAACATCAAAGACCAGAGGAC	NM_023960	107	58
		R:TGAGGACACCCACCACAAAC
Potassium intermediate/small-conductance calcium-activated channel, subfamily N, member 1 (SK1)	Kcnn1	F:AGCTCCGGACTGTGAAGATT	NM_019313	213	55
		R:TGGCTTGGGCTATGAGACTT
Potassium intermediate/small-conductance calcium-activated channel, subfamily N, member 2 (SK2)	Kcnn2	F:ACGCTAGTGGATCTGGCAAA	NM_019314	240	58
		R:ACGCTCAGCATTGTAGGTGA
Potassium intermediate/small-conductance calcium-activated channel, subfamily N, member 3 (SK3)	Kcnn3	F:CAGGAAACACCAGAGGAAGT	NM_019315	228	58
		R:AGGGAATTGAAGCTGGCTGT
Ribosomal protein L13a (Rpl13a)	Rpl13a	F:ACAAGAAAAAGCGGATGGTGf	NM_173340	172	58
		R:TTCCGGTAATGGATCTTTGCf
Hypoxanthine phosphoribosyltransferase 1 (HPRT)	Hprt1	F:TTGTTGGATATGCCCTTGACTg	NM_012583.2	105	58
		R:CCGCTGTCTTTTAGGCTTTGg

^aAbbreviation used in this article (abb.).

^bSequences of forward (F) and reverse (R) primers.

^cAccession number of GenBank (http://www.ncbi.nlm.nih.gov/).

^dAmplicon length in base pairs (bp).

^eAnnealing temperature (Tm).

^fSequences referenced to Bonefeld et al. (2).

^gSequences referenced to van Wijngaarden et al. (47).