Table 2.

Protein expression and phosphorylation immediately after exercise and before release of pressure cuff

	mTOR (Ser2448)	S6K1 (Thr389)	4E-BP1 (Thr37/46)	eEF2 (Thr56)	rpS6 (Ser235/236)	rpS6 (Ser240/244)	AMPKα (Thr172)	eIF2Bε (Ser539)	FAK (Tyr576/577)	ERK1/2 (Thr202/Tyr204)	Mnk1 (Thr197/202)	HIF-1α	REDD1	HSP70	IL-6	Akt (Thr308)	FOXO3a (Ser253)	IGF-I
Ctrl	0.84 ± 0.16	0.49 ± 0.21	0.47 ± 0.04*	1.94 ± 0.44	0.44 ± 0.13*	0.64 ± 0.35	0.45 ± 0.16	0.12 ± 0.06	0.97 ± 0.09	9.36 ± 3.87	0.12 ± 0.02*	1.30 ± 0.30	4.43 ± 0.44	0.60 ± 0.08	1.04 ± 0.22	2.57 ± 0.89	0.53 ± 0.07	0.91 ± 0.10
BFR	0.52 ± 0.09	0.31 ± 0.10*	0.63 ± 0.07	1.22 ± 0.08	0.89 ± 0.76	0.53 ± 0.27	0.36 ± 0.05	0.08 ± 0.02	1.27 ± 0.18	4.49 ± 1.41	0.06 ± 0.01†	1.19 ± 0.28	3.27 ± 0.36	0.78 ± 0.08	0.75 ± 0.07	2.43 ± 0.42	0.55 ± 0.06	0.92 ± 0.08

Values are means ± SE expressed in arbitrary units. mTOR, mammalian target of rapamycin; S6K1, S6 kinase 1; 4E-BP1, eukaryotic initiation factor 4E binding protein 1; eEF2, eukaryotic elongation factor 2; rpS6, ribosomal protein S6; AMPKα, AMP-activated protein kinase α; eIF2Bε, eukaryotic initiation factor 2Bε; FAK, focal adhesion kinase; ERK1/2, extracellular signal-regulated kinase 1/2; Mnk1, mitogen-activated protein kinase-interacting kinase 1; HIF-1α, hypoxia-inducible factor 1α; REDD1, regulated in development and DNA damage responses 1; HSP70, heat shock protein 70; IL-6, interleukin-6; FOXO3a, Forkhead Box O3a; IGF-I, insulin-like growth factor I. Proteins with an indicated phosphorylation site are measured as a phospho-to-total ratio. Proteins without a regulatory phosphorylation site are measured as total protein alone.

^*Significantly different from baseline (P < 0.05);

^†significantly different from Ctrl at same time point (P < 0.05).