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HCN subunit expression pattern in the rat cochlea at P9. Three-dimensional reconstruction of confocal images from cochlear whole-mount preparations, apical turns. A: HCN1 labeling (green). B: HCN4 labeling (green). Vesicular glutamate transporter VGLUT3 (red) was used as a marker for IHCs. HCN1 and HCN4 labeling was found in the inner spiral plexus (isp) under the row of IHCs (ihc, arrowhead) as well as in the somata of spiral ganglion neurons (sgn). Scale bars: 50 μm.
