Figure 2.
Representative spectra showing levels of peptides in wild-type and Cpefat/fat mice. Left panel: ions at m/z 420.483 and 425.010 (monoisotopic peaks) correspond to mono-D0- and D9- TMAB labeled RVD-hemopressin-α in olfactory bulb in an experiment evaluating WT vs WT mice. Ratio of D0 to D9 is 1.0, illustrating no change in peptide level in WT mice. Right panel: ions at 420.485 and 425.011 (monoisotopic peaks) correspond to mono-D0- and D9-TMAB labeled RVD-hemopressin-α in olfactory bulb in an experiment evaluating Cpefat/fat mice vs WT mice. Quantification of this spectra illustrates RVD-hemopressin-α is 5.5 fold higher in the Cpefat/fat mouse sample as compared to the WT mouse sample.