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Quantitative real-time PCR of hemoglobin mRNA levels in wild-type and Cpefat/fat mice. Results are shown as the fold-change in expression. Fold change was calculated using the ΔΔCt method, with GAPDH as an internal control. Both male and female mice used. n= 5 Cpefat/fat mice and 6 WT mice. Error bars represent standard error of the mean.
