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. 2010 Mar;27(3):565–585. doi: 10.1089/neu.2009.0966

FIG. 5.

FIG. 5.

MHC-II immunoreactivity overlapped that of pERK and was in proximity to that of GAP-43 within the LCST of the lower cervical spinal cord from the 1-m.p.l. adult rhesus monkey after injury of the lateral motor cortex. Transverse cervical spinal-cord sections from the 1-m.p.l. animal (SDM49) were processed for double immunofluorescent labeling against pERK (A, green) or GAP-43 (D, green) in conjunction with MHC-II (red, B, E). The merged images of pERK/MHC-II and GAP-43/MHC-II staining are shown in panels C and F respectively (MERGE). The blue fluorescence in panel C and F depicts nuclear labeling by DAPI. Photomicrographs were taken from the LCST region using a standard or confocal fluorescent microscope for pERK/MHC-II staining (A–C) and for GAP-43/MHCII (D–F) respectively. Note that pERK and MHC-II labels greatly overlap (C) while GAP-43 and MHC-II labels show little colocalization (F). Scale bar in panel C = 25 μm; scale bar in panel F = 20 μm.