Fig 3.
Local anaesthetics influence wound inflammation. (a) Day 3 wound numbers of Ly-6G-positive neutrophils in intact mice treated perioperatively with LA or vehicle (statistical significance tested by one-way anova [P<0.002], influence of treatment) followed by Bonferroni-corrected Student's t-tests: **P<0.01 (vs Con). (b) Transwell migration of IL-8-primed blood-derived neutrophils co-treated with lidocaine. (c) Day 3 wound numbers of MIF-positive cells (one-way anova). (d) Epidermal MIF staining scores (Kruskal–Wallis test). (e) MIF protein levels (immunoblotting) (values denote MIF:β-actin signal ratios). Values (a and c) are expressed as mean (sem). n=5–8 per treatment group. Arrows (a and c) identify cell immunostaining. Bup., bupivacaine; Con, control (vehicle: sterile dH2O); Ep., epidermal; Lid., lidocaine.