Figure 1.

β-Catenin signaling in cortical neural precursors. β-Catenin signaling in cortical ventricular zone revealed by expression of destabilized GFP controlled by TOP reporter elements. E13.5 precursors were electroporated with pTOP-dGFP and pCAG-RFP with either CMV stuffer plasmid (A) or DN-TCF4 (B), or ICAT (C), and examined after 24 h. Right, Higher magnification confocal images from the same sections shown in the left panels. Green signal representing activation of signaling by β-catenin is limited to cells in ventricular zone and eliminated by coelectroporation of DN-TCF4 or ICAT. Scale bars: left, 200 μm; right, 20 μm. IZ, Intermediate zone; CP, cortical plate.