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. 2006 Nov 29;26(48):12620–12630. doi: 10.1523/JNEUROSCI.3180-06.2006

Figure 2.

Figure 2.

β-Catenin signaling does not characterize a specific subpopulation of ventricular zone precursors. E13.5 ventricular zone precursors were electroporated by equal amounts of pCAGmRFP and pTOPdGFP plasmid. Twenty-four hours after electroporation, the vast majority of electroporated cells in the VZ were double-labeled with both RFP and GFP (representing activation of β-catenin signaling). An identical fraction of cells were labeled exclusively with either mRFP or GFP, indicating that TOPdGFP does not selectively label subpopulations of ventricular zone precursors. Cells exiting the ventricular zone [subventricular zone (SVZ)/intermediate zone (IZ)] retain RFP while downregulating GFP expression. The proportions of cells in the VZ exclusively labeled with RFP or GFP were not significantly different (n = 4 brains, 504 cells; ANOVA, p < 0.0001, with p > 0.05 for RFP-only cells compared with GFP-only cells by Tukey's post hoc test) for VZ cells. For non-VZ cells (n = 195), all proportions are significantly different from each other (ANOVA, p < 0.0001, with p < 0.001 for RFP-only cells compared with GFP-only cells). Error bars indicate SEM.