Figure 3.

β-Catenin signaling undergoes dynamic changes during neuronal differentiation. E13.5 ventricular zone precursors coelectroporated with equal amounts of pCAG-mRFP or pTOPdGFP were analyzed after different intervals. At 24 h, GFP expression, representing β-catenin signaling, is restricted to the ventricular zone. At 48 h, some postmitotic neurons have begun to migrate from the ventricular zone and continue to express RFP, although they have downregulated GFP as they enter the intermediate zone. Ventricular zone cells continue to express GFP. At 72 h, young neurons continuing to express RFP begin to arrive at the cortical plate. GFP expression is absent from the intermediate zone and cortical plate, and is only found rarely in the ventricular zone. At 96 h after electroporation, cortical plate neurons begin to reexpress GFP. Scale bar, 50 μm.