Figure 4.
β-Catenin protein is reduced after Cre-electroporation in floxed β-catenin mouse cortex. Floxed β-catenin cortical cells electroporated with Cre-Ires2-GFP have decreased immunoreactivity for β-catenin after 24 h (A) and 48 h (B). E13.5 timed-pregnant Flox βCat mice were electroporated with Cre-IRES2-EGFP (0.5 μg/μl) and mRFP1 (1.0 μg/μl). Twenty-four hours after surgery (E14.5), mice were killed and brains were removed from embryos, disassociated with trypsin, and plated on coverslips in six-well plates (5 × 106 cells/well). Cells were fixed 2 h after plating. Cells were stained for GFP and β-catenin (BCat) with antibodies. Immunofluorescent intensity for β-catenin was quantified, and cells expressing Cre-IRES2-EGFP were compared with cells exclusively expressing RFP. *p = 0.0312, ***p < 0.0001 by Student's t test. Error bars indicate SEM. Scale bar, 10 μm.