Skip to main content
. 2010 Apr 8;107(16):7455–7460. doi: 10.1073/pnas.1001006107

Fig. 1.

Fig. 1.

The human GLS2 gene contains a p53 DNA consensus binding element in the promoter region. (A) Putative p53 consensus binding elements in the human GLS2 gene predicted by the p53MH program. N, any nucleotide; Pu, purine; Py, pyramidine. (B) p53 binds to the p53 consensus binding element in the human GLS2 promoter region as detected by ChIP assays. The V138/H1299 cells were shifted to 32 °C for 24 h before assays. The p53-binding element in the MDM2 promoter serves as a positive control. DO-1: p53 antibody. (C) p53 activates luciferase activity of a reporter vector containing the p53-binding element in the GLS2 promoter region. The p53-null H1299 and HCT116 p53−/− cells were cotransfected with the luciferase reporter vectors and vectors expressing either wild-type (pRC p53) or mutant p53 protein (pRC 273H) 24 h before measuring luciferase activities.