Table 1.
Sequence changes in variants derived from Lib1-B7 upon directed evolution for higher foldability
| Lib1-B7(net) | |||||||||||||
| Position | 2 | 11 | 20 | 21 | 27 | 31 | 41 | 44 | 45 | 55 | 80 | 95 | |
| Residue | Glu | Asn | Lys | Ile | Asn | Phe | Tyr | Ser | Lys | Gln | Phe | Phe | |
| Round 1 variants * | 1 (SE1E7) | Asp | |||||||||||
| 2 (SE1E8) | Leu | ||||||||||||
| 3 (SE1D9) | Leu | ||||||||||||
| 4 (SE1F2) | Ser | ||||||||||||
| 5 (SE1A3) | Arg | ||||||||||||
| 6 (SE1C11) | Asp | ||||||||||||
| Round 2 variants | 7 (SE2E10) | Ser | Leu | ||||||||||
| 8 (SE2C4) | Ser | Asp | |||||||||||
| 9 (SE2A11) | Ser | Leu | |||||||||||
| 10 (SE2D9) | Asp | Tyr | Asn | ||||||||||
| 11 (SE2A8) | Ser | Leu | |||||||||||
| 12 (SE2F2) | Ser | Leu | |||||||||||
| Round 3 variants | 13 (SE3C11) | Ser | Leu | ||||||||||
| 14 (SE3G8) | Asp | Tyr | Asn | Leu | |||||||||
| 15 (SE3G11) | Ser | Glu | Asp | Asn | |||||||||
| Round 4 variants | 16 (SE4B2) | Gly | Ser | Glu | Asp | Asn | Arg | Leu | |||||
| 17 (SE4H3) | Ser | Glu | Asp | His | Arg | Asn | Leu | ||||||
| 18 (SE4E11) † | Ser | Glu | Asp | Asn | |||||||||
| 19 (SE4H12) | Ser | Glu | Val | Asp | Asn | Leu | |||||||
*For simplicity, the evolved variants derived from the wild-type fragment Lib1-B7 were sequentially numbered as Lib1-B7-1 to Lib1-B7-19. The original annotations of these variants are given in parenthesis.
†Variant Lib1-B7-18, the structure of which is available and its complete amino acid sequence is: MEKGTPPTHDSDNWMGRAKEIGNGGWDQFQFLFFDPNGYLYAVSNDKLYKASPPQSDTDNWIARATEIGSGGWSGFKFLFFHPNGYLYAVRGQRFYKALPPVSNQD