Table 2.
Relative amounts of mRNAs in livers of calorie-restricted WT and Goat−/− mice
| mRNA | ad libitum fed | Calorie-restricted | ||
| WT | Goat−/− | WT | Goat−/− | |
| SREBP pathway | ||||
| SREBP-1c | 1.0 | 0.85 | 0.08 | 0.08 |
| SREBP-2 | 1.0 | 1.07 | 0.48 | 0.38 |
| Lipogenesis | ||||
| ATP citrate lyase | 1.0 | 1.32 | 0.13 | 0.13 |
| ACC-1 | 1.0 | 1.10 | 0.50 | 0.51 |
| FAS | 1.0 | 1.33 | 0.14 | 0.14 |
| SCD-1 | 1.0 | 1.25 | 0.40 | 0.52 |
| Gluconeogenesis | ||||
| PEPCK | 1.0 | 1.08 | 4.46 | 4.78 |
| G6Pase | 1.0 | 1.05 | 2.84 | 3.82 |
| Others | ||||
| IGF1 | 1.0 | 1.12 | 0.41 | 0.38 |
| IGFBP-1 | 1.0 | 0.89 | 36.7 | 74.5 |
| IGFBP-3 | 1.0 | 1.65 | 0.76 | 0.66 |
Male WT and Goat−/− littermates (8-wk-old) were fed a chow diet ad libitum or subjected to 60% calorie restriction for 7 days and euthanized at 5:30 p.m. Total RNA from four to six mouse livers per group were pooled and quantified by real-time PCR as described in Materials and Methods. Glyceraldehyde 3-phosphate dehydrogenase mRNA was used as the invariant control. Each value represents the amount of mRNA relative to that in fed WT mice, which is arbitrarily defined as 1.0. Primers for real-time PCR analysis were as described in Materials and Methods except for the following: IGF1 (NM_010512): 5′-CCACACTGACATGCCCAAGA-3′ and 5′-TCCTTTGCAGCTTCGTTTTCT-3′; IGFBP-3 (NM_008343): 5′-GAGTGTGGAAAGCCAGGTT-GTC-3′ and 5′-GCATGGAGTGGATGGAACTTG-3′. SREBP, sterol regulatory element-binding protein; ACC-1, acetyl-CoA carboxylase-1; FAS, fatty acid synthase; SCD-1, stearoyl-CoA desaturase-1; PEPCK, phosphoenolpyruvate carboxylase; G6Pase, glucose-6-phosphatase.