Fig. 3.

In vitro phosphorylation of ideal (WT) and substituted peptides by the six kinases that share a substrate phosphorylation site motif. A peptide from Rv0497 that has features of the shared phosphorylation site motif was synthesized, together with 31 additional peptides incorporating substitutions predicted to enhance or diminish phosphorylation. The phosphoacceptor is shown in bold. Substituted residues are underlined. Phosphorylation of each peptide is expressed as a ratio relative to phosphorylation of the original peptide (WT). Substitutions that increase or decrease phosphorylation by 50% or more are shaded in green or red, respectively. Mean values of two independent experiments are shown.