Fig. 2.

Diversity of the human fecal microbiome and its metatranscriptome. (A) Distribution of gene clusters across gut microbial genomes and microbiome bins. All protein sequences from 122 gut genomes and the microbiome bins were clustered using cd-hit at 60%ID. (B) Number of sequence variants in each microbiome bin (values normalized by Gbp in bin; all genus-level bins with >100 scaffolds are shown). (C) Rarefaction analysis of the number of genes, gene clusters, expressed genes, and expressed gene clusters in the fecal microbial communities of TS28 and TS29 as a function of sequencing depth. The total number of protein-coding genes in the set of 122 gut genomes and the microbiome bins is 525,329, representing 257,823 gene clusters. (D) Ratio of gene expression to gene abundance (relative abundance of cDNA sequences divided by relative abundance of DNA sequences) mapped to a subset of the bacterial taxa in the fecal microbiome. Taxa with >1,000 mapped cDNA and DNA sequencing reads in both samples are shown.