Fig. 2.

Multiple sequence alignment of the N-terminal regions of 10 representative PduP homologues from different organisms. Terminal sequence extensions are apparent in four of the five representatives whose genomic context suggests an association with MCP function (BMC-proximal). PduP homologues belong to the conserved NAD(P)⁺-dependent aldehyde dehydrogenase superfamily (cl11961), whose members oxidize a range of aldehyde substrates in distinct metabolic pathways. The representative homologues were selected from clusters of similar sequences from an alignment of some 100 distinct sequences (the number of sequences in each cluster follows the organism name in parentheses). The National Center for Biotechnology Information gene accession identifications, beginning from the top entry, are 5069459, 16761381, 46907383, 123442957, 188587712, 27366378, 50121254, 110798574, 114563069, and 148378348. The full alignment is provided in Dataset S1. N-terminal methionine residues were omitted to prevent spurious alignment of the divergent termini.