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Association of PduP with purified Pdu MCPs. (A) SDS/PAGE: (lane 1) WT S. enterica, (lane 2) ΔpduP/pLac22-no insert, (lane 3) ΔpduP/pLac22-pduP1–464 (full-length PduP), (lane 4) ΔpduP/pLac22-pduP11–464, and (lane 5) ΔpduP/pLac22-pduP15–464. Ten micrograms of MCPs was loaded in each well. (B) PduP enzyme activity for the starting cell extracts (dark gray) and purified MCPs (light gray) analyzed in A.
