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Western blots for eGFP, GST, and fusion proteins. (A) Western blot for eGFP. MCPs purified from (left to right) WT S. enterica, ΔpduP/pLac22-eGFP, ΔpduP/pLac22-PduP1–10-eGFP, ΔpduP/pLac22-PduP1–14-eGFP, ΔpduP/pLac22-PduP1–18-eGFP, and ΔpduP/pLac22-PduP1–70-eGFP. (B) Western blot for GST. MCPs purified from (left to right) WT S. enterica, ΔpduP/pLac22-GST, ΔpduP/pLac22-PduP1–10-GST, ΔpduP/pLac22-PduP1–14-GST, ΔpduP/pLac22-PduP1–18-GST, and ΔpduP/pLac22-PduP1–70-GST.
