Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Competition between fusion proteins and PduP for MCP binding. (A) Increasing IPTG concentrations (0, 0.01, 0.1, and 1 mM) were used to increase production of PduP1–18-GFP by S. enterica/pLac22-PduP1–18-eGFP. MCPs were purified, and Western blots were used to detect GFP and PduP. (B) Amount of PduP and eGFP associated with MCPs purified from S. enterica/pLac22-PduP1–18-eGFP grown with different IPTG concentrations based on PduP enzyme activity and relative fluorescence.
