Fig. 6.
Western blot for eGFP following immunoprecipitation of eGFP from broken and intact MCPs. MCPs were purified from ΔpduP/plac22-PduP1–18-eGFP, and a portion was broken by dialysis and sonication. Rabbit anti-GFP was incubated with broken and intact MCPs and then precipitated with protein A-immobilized beads. The proteins that coprecipitated with the beads and the supernatant fractions were analyzed by Western blot for eGFP. IgG was also detected by the secondary antibody used for the Western blot.