Fig. 4.

(A) Time-resolved SAXS/WAXS differences. For clarity, the curves are color-coded according to the model in Fig. 1B and offset from one another. (B) Time-independent scattering fingerprints extracted from global analysis of the time-resolved scattering data in A. Scattering differences between each intermediate state and the ground state (MbCO) are plotted as solid lines, whereas differences between each state and the state that precedes it are plotted as dotted lines (three-point smoothing has been applied to the dotted lines). For clarity, the curves are offset vertically from one another. A scaled thermal signal from static measurements (gray) is plotted on top of the thermal signal recovered from global analysis of the time-resolved scattering patterns. (C, Upper) Time-dependence of the integrated SAXS signal. (Lower) Time-dependent population of states in B. The dashed line labeled IRF (cyan) represents the instrument response function (convolution of the laser and x-ray pulses).