Fig. 6.

Blocking E selectin and P selectin in vivo reduces DETC number in neonatal skin. (A) Experimental design. From ED14 to ED20 (birth), the pregnant WT C57BL/6 mice were injected i.p. daily with combined mAbs (100 μg or 50 μg each) against E selectin and P selectin or with isotype-matched control mAbs. The neonatal back skins were harvested for the preparation of single-cell suspensions by enzymatic digestion. (B) Cells then were labeled for DETC frequency analysis by flow cytometry. (C) Summary data on the percentages of DETC in neonatal skin after blocking E selectin and P selectin. **, P < 0.01. Results are representative of two independent experiments.