Fig. 7.

DETC are diminished in CCR4^−/− but not in CCR10^−/− mice. (A) Epidermal cells were prepared from 8-week-old WT, CCR4^−/−, or CCR10^−/− C57BL/6 mice. Cells then were washed thoroughly and labeled with fluorescence-conjugated anti-CD3 and anti-Vγ3 TCR mAbs for DETC analysis by flow cytometry. (B) Summary data on the percentages of DETC in CD3⁺ T cells. **, P < 0.01. (C) DETC on epidermal sheets were visualized by labeling with FITC-conjugated anti-CD3 or anti-Vγ3 TCR mAb (×100). One of three independent experiments with similar results is shown.