Table 2.
New proteins expressed after the 12th culture passage.
| Spot # | Abbr. Name | ACa | Protein Name | Scoreb | SCc % | pI | Mr(Da) Theo. | pI | Mr(Da) Exp. |
|---|---|---|---|---|---|---|---|---|---|
| 5 | VIME | P08670 | Vimentin | 124 | 58 | 5,06 | 53651 | 5,09 | 45706 |
| 16 | P4HA2 | O15460 | Prolyl 4-hydroxylase subunit alpha-2 | 64 | 53 | 5,50 | 61623 | 5,50 | 61292 |
| 17 | ERO1A | Q96HE7 | Oxidoreductin-1-Lalpha | 98 | 71 | 5,43 | 55213 | 5,65 | 58342 |
| 31 | SYDC | P14868 | Aspartyl-tRNA synthetase, cytoplasmic | 89 | 67 | 6,10 | 57700 | 6,09 | 57478 |
| 43 | P4HA1 | P13674 | Prolyl 4-hydroxylase subunit alpha-1 | 101 | 45 | 5,70 | 61157 | 5,12 | 62401 |
A) Entry code number from SWISS PROT database.
B) Score is -10*Log(P), where P is the probability that the observed match is a random event, it is based on Swiss-Prot database using the MASCOT searching program as MALDI-TOF data.
C) Sequence coverage means the ratio of portion sequence covered by matched peptide to the full length of the protein sequence.