Table 2.
The regulation of transcripts and proteins of the polyamine pathway in three distinct inhibition experiments.
| Annotation | PlasmoDB ID | Cyclohexylamine (PfSpdSyn inhibition) (This work) |
DFMO/MDL73811 (PfAdoMetDC/ODC inhibition) [9] |
DFMO (ODC inhibition) [28] |
||
|---|---|---|---|---|---|---|
| Transcript | Protein | Transcript | Protein | Transcript | ||
| Ornithine aminotransferase | PFF0435w | ≈ | ↓ | ↑ | ↑ | ↑ |
| S-adenosylmethionine decarboxylase-ornithine decarboxylase (PfAdoMetDC/ODC) | PF10_0322 | ≈ | nd | ↓ | nd | nd |
| Hypoxanthine phosphoribosyltransferase | PF10_0121 | ≈ | nd | ≈ | nd | ↑ |
| Uridine phosphorylase, putative | PFE0660c | ↓ | ↓ | ↓ | nd | nd |
| Adenosine deaminase, putative | PF10_0289 | ↓ | ↓ | ↓ | nd | nd |
| Spermidine synthase | PF11_0301 | ≈ | nd | ≈ | nd | nd |
| S-adenosylmethionine synthetase, putative | PFI1090w | ↓* | ↓ | ↓ | ↓ | nd |
| Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase) | PFE1050w | ↓* | nd | ↓ | nd | nd |
| Lysine decarboxylase, putative | PFD0285c | ↑ | nd | ↑ | nd | nd |
* = fold change between -1.5 and -2 fold
↓ = downregulated
↑ = upregulated
≈ = unchanged
nd = not determined
A large degree of overlap was observed for the response to polyamine depletion, whether PfSpdSyn, PfAdoMetDC/ODC or ODC alone were inhibited. Also, effects specific to each inhibitor could be gauged.