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Establishment of iPS cells from mouse models of Fabry, GLD, and MPSVII. Mouse iPS cells were established by retroviral transduction of Oct3/4, Sox2, and Klf4 into tail-tip fibroblasts. (A) iPS cells were grown on feeder cells and exhibited ES cell–like morphology. (B and C) All of the iPS clones analyzed were positive for SSEA1 (B, Left, phase contrast microscopy; Right, SSEA1 immunostaining) and alkaline phosphatase (C).
