Fig. 5.

In vivo multichannel FMT/PET-CT. FMT/PET-CT of tumor-bearing mice, coinjected with a fluorochrome-derived RGD peptide targeting integrin (Integrisense), a protease sensor (Prosense), and the nanoparticle PET agent (⁶⁴Cu-CLIO-VT680). (A and B) In 2D images, FMT (A) and PET (B) signal intensities were color-coded, whereas 3D reconstructions show a surface-rendered signal. (C) Overall, the signals in all FMT channels and in PET colocalized in tumors; however, when assessed at higher magnification, distinct differences between probes due to their different target profiles became evident.