Fig. 4.

Full-length IL-33 is abundantly expressed, whereas ST2L is decreased in IEC from UC patients. IEC were harvested from full-thickness inflamed colons of UC (n = 18) and CD (n = 10) and noninflamed CON (n = 7) patients. Total RNA and protein were extracted and processed for RT-PCR and Western blots. (A) IL-33 (Upper) and ST2 (Lower) mRNA levels. Statistical analysis was performed by using one-way ANOVA. (B) Representative Western blots of 4 separate experiments for IL-33 (Upper) and ST2 (Lower) on IEC from inflamed IBD colons and noninflamed CON. β-actin was used as an internal housekeeping control.