Fig. 1.

Expression of PNPLA3 in human tissues (A) and mouse liver (B). (A) Relative levels of PNPLA3 and cyclophilin mRNA were determined by quantitative real-time PCR using cDNA from 48 human tissues (Origene). The cDNAs were standardized using cyclophilin as a calibrator. Each bar represents the mean of triplicate measurements expressed as a fraction of the Ct value obtained from liver, which was set to 1. (B) The relative levels of mRNA from genes expressed predominantly in hepatocytes [albumin (ALB) apolipoprotein B (APOB)] and stellate cells [glial fibrillary acidic protein (GFAP), lecithin retinol acyltransferase (LRAT)]. The hepatocytes and stellate cells were fractionated from mouse liver and mRNA levels were quantitated using quantitative real-time PCR as described in Materials and Methods.